EpCAM Antibody / Cytoplasmic domain

SKU: orb749649

Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Canine, Feline, Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: EGP40/1110
• Immunogen: A portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the EpCAM antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EpCAM antibody (clone EGP40/1110).

IHC: Formalin-fixed, paraffin-embedded rat Oviduct stained with EpCAM antibody (clone EGP40/1110).

IHC: Formalin-fixed, paraffin-embedded rat colon stained with EpCAM antibody (clone EGP40/1110).

IHC: Formalin-fixed, paraffin-embedded rat lung stained with EpCAM antibody (clone EGP40/1110).

IHC testing of FFPE mouse colon tissue with EpCAM antibody (clone EGP40/1110). Required HIER: boil tissue sections in 10mM Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EpCAM antibody (clone EGP40/1110). These results demonstrate the foremost specificity of the EGP40/1110 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone EGP40/1110) as confirmation of integrity and purity.

Quick Database Links

UniProt

P16422