EPB41L2 Antibody

• Catalog Number: orb1247501
• Category: Antibodies
• Description: EPB41L2 Antibody
• Target: EPB41L2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-RREVRSPTKAPH
• UniProt ID: O43491
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 113kDa according to NP_001422.1). Recommended concentration: 0.03-0.1ug/ml. An additional double band of unknown identity was also consistently observed at 38-40kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Uterus shows membranous staining of the endometrial cells. Recommended concentration, 3-5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EPB41L2, 4.1-G, erythrocyte membrane protein band
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001422.1

orb1247501 (0.1 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247501 (2.5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.