Eotaxin (CCL11) Antibody

• Catalog Number: orb1671465
• Category: Antibodies
• Description: Eotaxin (CCL11) Antibody
• Target: CCL11
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: Eotaxin specific antibody was purified by affinity chromatography employing immobilized hEotaxin matrix
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEotaxin. Anti-Human Eotaxin specific antibody was purified by affinity chromatography employing immobilized hEotaxin matrix.
• UniProt ID: P51671
• Tested applications: ELISA, WB
• Application notes: Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Eotaxin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin. Western Blot To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CCL11
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: P51671

To detect hEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Eotaxin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin.

To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.