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Eosinophil Peroxidase Antibody

Catalog Number: orb699615

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb699615
CategoryAntibodies
DescriptionPeripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
ClonalityRecombinant
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenHuman eosinophils from a patient with hypereosinophilic syndrome were used as the immunogen for the recombinant Eosinophil Peroxidase antibody.
UniProt IDP11678
Tested applicationsFACS, IF, IHC-P
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
Application notesOptimal dilution of the recombinant Eosinophil Peroxidase antibody should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberrEPO104
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis recombinant Eosinophil Peroxidase antibody is available for research use only.
NoteFor research use only
Eosinophil Peroxidase Antibody

IHC staining of FFPE human pancreas with recombinant Eosinophil Peroxidase antibody (clone rEPO104). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Eosinophil Peroxidase Antibody

SDS-PAGE analysis of purified, BSA-free recombinant Eosinophil Peroxidase antibody (clone rEPO104) as confirmation of integrity and purity.

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    IHC-P,  WB

    Guinea pig, Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μg, 100 μg
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    IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 200 μl, 100 μl
  • EPX Antibody [orb417715]

    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • EPX Rabbit Polyclonal Antibody [orb5168]

    IF,  IHC-Fr,  IHC-P

    Rat

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 200 μl, 100 μl
  • Eosinophil Peroxidase Antibody [orb699616]

    FACS,  IF,  IHC-P

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg, 100 μg