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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD31413 |
| Immunogen | This ENPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-930 amino acids from the C-terminal region of human ENPEP. Antigen Region: 899-930 aa. |
| Target | ENPEP |
| Molecular Weight | 109244 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Glutamyl aminopeptidase, EAP, Aminopeptidase A, AP-A, Differentiation antigen gp160, CD249, ENPEP
Quality Guarantee
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ENPEP Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the ENPEP antibody detected the ENPEP protein (arrow).
ENPEP Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ENPEP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ENPEP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001968.3 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ENPEP Antibody (C-term) (orb1937737)
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