Endothelin 3/EDN3 Antibody

• Catalog Number: orb1535593
• Category: Antibodies
• Description: Endothelin 3/EDN3 Antibody
• Target: Endothelin 3 / EDN3
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Purification: Purified from ascites by Protein A
• Immunogen: EDN3 (AAH08876, aa 1-238). A full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Tested applications: ELISA, IHC, IHC-P, IP, WB
• Dilution range: ELISA, IHC, IHC-P (10 µg/ml), IP, WB
• Application notes: Further information: Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
• Clone Number: 2A6-2A4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EDN3, Endothelin-3, ET3, Endothelin 3, Preproendot
• Note: For research use only

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western Blot analysis of EDN3 expression in transfected 293T cell line by EDN3 monoclonal antibody (M01), clone 2A6-2A4.Lane 1: EDN3 transfected lysate (25.5 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation of EDN3 transfected lysate using anti-EDN3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN3 rabbit polyclonal antibody.

Detection limit for recombinant GST tagged EDN3 is approximately 0.3 ng/ml as a capture antibody.