Endothelin 3/EDN3 Antibody

SKU: orb1535593

Description

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, IHC-P, IP, WB
Dilution Range	ELISA, IHC, IHC-P (10 µg/ml), IP, WB
Reactivity	Human
Application Notes	Further information: Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1,k
Clone No.	2A6-2A4
Immunogen	EDN3 (AAH08876, aa 1-238). A full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Target	Endothelin 3 / EDN3
Purification	Purified from ascites by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, pH 7.4
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EDN3, Endothelin-3, ET3, Endothelin 3, Preproendothelin-3, WS4B, ET-3, HSCR4, PPET3

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western Blot analysis of EDN3 expression in transfected 293T cell line by EDN3 monoclonal antibody (M01), clone 2A6-2A4.Lane 1: EDN3 transfected lysate (25.5 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation of EDN3 transfected lysate using anti-EDN3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN3 rabbit polyclonal antibody.

Detection limit for recombinant GST tagged EDN3 is approximately 0.3 ng/ml as a capture antibody.