Endophilin B1 Antibody (Y80)

• Catalog Number: orb1928665
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB15350
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 40796 Da
• Target: This Endophilin B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from human Endophilin B1.
• UniProt ID: Q9Y371
• NCBI: NP_001193582.1, NP_057093.1, NP_001193580.1, NP_001193581.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Endophilin-B1, Bax-interacting factor 1, Bif-1, SH
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-Endophilin B1 Antibody (Y80) in HepG2 cell line lysates (35 ug/lane). SH3GLB1 (arrow) was detected using the purified Pab.

Anti-Endophilin Antibody (Y80) at 1:1000 dilution + U118MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.