EndoPDI Antibody

• Catalog Number: orb1247558
• Category: Antibodies
• Description: EndoPDI Antibody
• Target: TXNDC5
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SLHRFVLSQAKDEL
• UniProt ID: Q8NBS9
• Tested applications: ELISA, FC, IF, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6kDa according to NP_110437.2). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TXNDC5, EndoPDI, thioredoxin domain containing 5,
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001139021.1, NP_110437.2

orb1247558 staining (0.1 ug/ml) of HEK293 (A), A549 (B), HeLa (C) and (0.01 ug/ml) of HepG2 (D) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247558 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247558 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing endoplasmic reticulum staining.

orb1247558 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing endoplasmic reticulum staining.

orb1247558 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).