ENDOD1 Polyclonal Antibody

• Catalog Number: orb1419997
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ENDOD1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat
• Conjugation: Unconjugated
• UniProt ID: O94919
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Endonuclease domain-containing 1 protein, 3.1.30.-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: U251 Cell (Human) Lysate at 40 ug. Primary: Anti-ENDOD1 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 53kD. Observed band size: 53kD.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ENDOD1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.