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Catalog Number | orb1533728 |
---|---|
Category | Antibodies |
Description | Encephalopsin/OPN3 Antibody |
Target | Encephalopsin / OPN3 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human, Monkey, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide |
Immunogen | Synthetic 16 amino acid peptide from internal region of human OPN3 / Encephalopsin. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat (100%); Mouse, Dog, Panda, Rabbit (94%); Horse, Opossum (88%); Turkey (81%). |
Tested applications | ELISA, IHC, IHC-P |
Dilution range | ELISA, IHC, IHC-P (5 µg/ml) |
Application notes | Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | OPN3, ERO, Encephalopsin, NMO-1, Opsin 3, Opsin-3, Read more... |
Note | For research use only |