ENASE Antibody (Center)

• Catalog Number: orb1787883
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 326-354 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 0.25, IHC-P: 1:50~100, FC: 1:10~50
• Conjugation: Unconjugated
• MW: 83987 Da
• Target: This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
• UniProt ID: Q8NFI3
• NCBI: NP_001036038.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cytosolic endo-beta-N-acetylglucosaminidase, ENGas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ENASE Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-ENASE Antibody (Center) at 1:2000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: mouse testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ENASE Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.