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Catalog Number | orb383774 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against ENaC beta conjugated to APC. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 7B8 |
Tested applications | ICC, IF, IHC |
Reactivity | Mouse |
Isotype | IgG1 |
Immunogen | Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638) |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000); IHC (1:150) |
Conjugation | APC |
MW | 87kDa |
Target | ENaC beta |
Entrez | 24767 |
UniProt ID | P37090 |
NCBI | NP_036780 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
Alternative names | SCNN1B antibody, Amiloride sensitive sodium channe Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SMC-240 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.
Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.
ICC, IF, IHC | |
Frog, Hamster, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC |
ICC, IF, IHC | |
Frog, Hamster, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC |
FC | |
Bovine, Canine, Porcine, Rat | |
Guinea pig, Human, Mouse | |
Rabbit | |
Polyclonal | |
APC |
FC, ICC | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
APC |