ENaC alpha Antibody

SKU: orb67567

Description

Rabbit Anti-Rat ENaC alpha Polyclonal

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), ICC/IF (1:400); IHC-P (1:25)
• Reactivity: Frog, Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of alpha-ENaC in 35 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against a synthetic peptide mapping to the N-terminal of the alpha subunit (amino acids 46-68) of rat Alpha ENaC (antibody designation 3560-2).
• Target: ENaC alpha
• Molecular Weight: 85kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha ENaC, Alpha ENaC 2, Alpha NaCH, Alpha-ENaC, Amiloride sensitive epithelial sodium channel alpha subunit, Amiloride sensitive sodium channel subunit alpha, Amiloride-sensitive sodium channel subunit alpha, ENaCa, ENaCalpha, Epithelial Na(+) channel subunit alpha, Epithelial Na+ channel subunit alpha, Epithelial Sodium Channel alpha, Epithelial Sodium Channel-α, FLJ21883, Nonvoltage gated sodium channel 1 subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN 1, SCNN1, SCNN1A, SCNNA_HUMAN, Sodium channel nonvoltage gated 1 alpha

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) ENaC Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

ENaC alpha

UniProt

Q6IRJ1

NCBI Gene ID

25122

RefSeq (Protein):NP_113736