ENaC alpha Antibody

SKU: orb383746

Description

Mouse Anti-Rat ENaC alpha Monoclonal IgG2b

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1:1000); IHC-P (1:150)
• Reactivity: Mouse, Rat
• Application Notes:
  A 1:1000 dilution was sufficient for detection of ENaC alpha in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 2G4
• Immunogen: Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
• Target: ENaC alpha
• Molecular Weight: 85kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha ENaC, Alpha ENaC 2, Alpha NaCH, Alpha-ENaC, Amiloride sensitive epithelial sodium channel alpha subunit, Amiloride sensitive sodium channel subunit alpha, Amiloride-sensitive sodium channel subunit alpha, ENaCa, ENaCalpha, Epithelial Na(+) channel subunit alpha, Epithelial Na+ channel subunit alpha, Epithelial Sodium Channel alpha, Epithelial Sodium Channel-α, FLJ21883, Nonvoltage gated sodium channel 1 subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN 1, SCNN1, SCNN1A, SCNNA_HUMAN, Sodium channel nonvoltage gated 1 alpha

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.

Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.

Quick Database Links

Gene Symbol

ENaC alpha

UniProt

Q6IRJ1

NCBI Gene ID

25122

RefSeq (Protein):NP_113736