EMT Research Antibody Assembly kit (E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1)

SKU: orb1015983

Description

EMT Research Antibody Assembly kit (E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1)

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

EMT Research Antibody Assembly kit(E-Cadherin; N-Cadherin; Vimentin; Occludin; ZO-1)

Primary: Anti-Vimentin (orb11559), Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (orb11559) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Primary: Anti-ZO-1, Independently Validated Antibody. Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated at 1:250 followed by conjugation to the secondary antibody.

Primary: Anti-N-cadherin, Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Primary: Anti-Occludin, Paraformaldehyde-fixed, paraffin embedded (Normal skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.