EMI1 Antibody (Early Mitotic Inhibitor-1)

• Catalog Number: orb749516
• Category: Antibodies
• Description: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EMI1/1176
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.
• Dilution range: Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This EMI1 antibody is available for research use only.
• UniProt ID: Q9UKT4
• Storage: Store the EMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the EMI1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.