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Catalog Number | orb1930016 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19614 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 31376 Da |
Target | This ELOVL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-58 amino acids from the N-terminal region of human ELOVL6. |
UniProt ID | Q9H5J4 |
NCBI | NP_076995.1, NP_001124193.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Elongation of very long chain fatty acids protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ELOVL6 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ELOVL6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ELOVL6 antibody (N-term) in mouse liver tissue lysates (35 ug/lane). ELOVL6 (arrow) was detected using the purified Pab.
Anti-ELOVL6 Antibody (N-term) at 1:1000 dilution + human lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.