ELMO3 Antibody

SKU: orb1247560

Description

ELMO3 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 70.0kDa according to NP_ 078988.2). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:In paraffin embedded Human Prostate shows vesiculate staining in the cytoplasm of secretory cells. Recommended concentration, 5-10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: TNFNFCYDCSIAEP
• Target: ELMO3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ELMO3, engulfment and cell motility 3 (ced-12 homolog, C. elegans), CED12, FLJ13824, ced-12 homolog 3, engulfment and cell motility 3, CED-12, ELMO-3

orb1247560 staining (0.1 ug/ml) of Human Brain (Hipoocampus) lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247560 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ELMO3

UniProt

Q96BJ8

RefSeq (Protein):NP_078988.2