ELMO1 (vPairTM) Antibodies

SKU: orb131825

Description

Rabbit polyclonal to ELMO1. ELMO1 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Engulfment and cell motility protein 1 (ELMO1) is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. ELMO1 acts in assocation with DOCK1 and CRK. ELMO1 was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. Furthermore, ELMO1 may enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB:1:1000-1:3000
• Application Notes:
  WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal or the C-terminal region of human ELMO1.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: N-terminal or the C-terminal region of human ELMO1
• Molecular Weight: 84
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Engulfment and cell motility protein 1 antibody pairs, anti Protein ced-12 homolog antibody pairs

Quick Database Links

UniProt

Q92556