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| Catalog Number | orb767963 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Elf-1 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ELF1. AA range:537-586 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 67 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-ELF1 antibody, anti-ETS-related transcription Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of 293 cells using Elf-1 Polyclonal Antibody diluted at 1:1000.
Immunofluorescence analysis of A549 cells, using ELF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 and HUVEC cells, using ELF1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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