ELAVL2 Antibody (Center)

SKU: orb1937068

Description

ELAVL2 Antibody (Center)

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: IHC-P - 1:100, WB - 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat, Xenopus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 39504 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

HUB

Anti-ELAVL2 Antibody (Center) at 1:1000 dilution + human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ELAVL2 Antibody (Center) at 1:2000 dilution + Mouse brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ELAVL2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ELAVL2 Antibody (Center) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the ELAVL2 antibody detected the ELAVL2 protein (arrow).

Immunohistochemical analysis on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis on paraffin-embedded Human cerebellum tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Quick Database Links

UniProt

Q12926