EIF4G1 Antibody

SKU: orb1256320

Description

EIF4G1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
• Target: EIF4G1
• Molecular Weight: Observed: 230kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, OCT3, OCT4, OTF3,

Western blot analysis of extracts of THP-1 cells, using EIF4G1 Antibody (orb1256320) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver damage using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using EIF4G1 antibody (orb1256320). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

EIF4G1

UniProt

Q04637