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EIF4G1 Antibody
SKU: orb1256320
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3). |
| Target | EIF4G1 |
| Molecular Weight | Observed: 230kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, OCT3, OCT4, OTF3,
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Western blot analysis of extracts of THP-1 cells, using EIF4G1 Antibody (orb1256320) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF4G1 antibody (orb1256320). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
EIF4G1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
EIF4G1 Antibody (orb1256320)
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