EIF4G1 Antibody

• Catalog Number: orb1256320
• Category: Antibodies
• Description: EIF4G1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 230kDa
• Target: EIF4G1
• UniProt ID: Q04637
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, OCT3,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of THP-1 cells, using EIF4G1 Antibody (orb1256320) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver damage using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Antibody (orb1256320) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using EIF4G1 antibody (orb1256320). Blue: DAPI for nuclear staining.