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Item 1 of 4
EIF4B Antibody (Center)
Catalog Number: orb1939532
| Catalog Number | orb1939532 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB11720 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 69151 Da |
| Target | This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-382 amino acids from the Central region of human EIF4B. |
| UniProt ID | P23588 |
| NCBI | NP_001408.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Eukaryotic translation initiation factor 4B, eIF-4 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-EIF4B Antibody (Center) Pab in HepG2 cell line lysates (35 ug/lane). EIF4B (arrow) was detected using the purified Pab.
Western blot analysis of anti-EIF4B Antibody (Center) Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2 ug/lane). EIF4B (arrow) was detected using the purified Pab.
Anti-EIF4B Antibody (Center) at 1:1000 dilution + Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
