EIF4A1 Antibody

SKU: orb1257576

Description

EIF4A1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:20 - 1:50IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1).
• Target: EIF4A1
• Molecular Weight: Observed: 46kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I, eukaryotic initiation factor 4A-I, ATP-dependent RNA helicase eIF4A-1, eukaryotic initiation factor 4AI, eukaryotic translation initiation factor 4A

Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (orb1257576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody (orb1257576). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of NIH/3T3 cells using 3 ug EIF4A1 antibody (orb1257576). Western blot was performed from the immunoprecipitate using EIF4A1 antibody (orb1257576) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

EIF4A1

UniProt

P60842