EIF4A1 Antibody

• Catalog Number: orb1257576
• Category: Antibodies
• Description: EIF4A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:20 - 1:50IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 46kDa
• Target: EIF4A1
• UniProt ID: P60842
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I, eukaryoti
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:20 - 1:50IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (orb1257576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody (orb1257576). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of NIH/3T3 cells using 3 ug EIF4A1 antibody (orb1257576). Western blot was performed from the immunoprecipitate using EIF4A1 antibody (orb1257576) at a dilution of 1:1000.