EIF3D Antibody

• Catalog Number: orb1257241
• Category: Antibodies
• Description: EIF3D Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human EIF3D (NP_003744.1).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 64kDa
• Target: EIF3D
• UniProt ID: O15371
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: EIF3D, EIF3-zeta, EIF3 p66, EIF3-p66, EIF-3-zeta,
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EIF3D antibody (orb1257241) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of L-929 cells using EIF3D antibody (orb1257241) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using EIF3D antibody (orb1257241) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L-929 cells using EIF3D antibody (orb1257241) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using EIF3D antibody (orb1257241) at dilution of 1:100. Blue: DAPI for nuclear staining.