EIF2AK4 Antibody

SKU: orb1260821

Description

EIF2AK4 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).
• Target: EIF2AK4
• Molecular Weight: Observed: 187kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EIF2AK4, GCN2, GCN2 eIF2alpha kinase, GCN2-like protein, Mgcn2, KIAA1338

Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (orb1260821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (20x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (orb1260821). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

EIF2AK4

UniProt

Q9P2K8