EIF2AK4 Antibody

• Catalog Number: orb1260821
• Category: Antibodies
• Description: EIF2AK4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 187kDa
• Target: EIF2AK4
• UniProt ID: Q9P2K8
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: EIF2AK4, GCN2, GCN2 eIF2alpha kinase, GCN2-like pr
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (orb1260821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (20x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (orb1260821). Blue: DAPI for nuclear staining.