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| Catalog Number | orb764179 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to eIF2alpha (phospho-Ser51) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Canine, Fish, Human, Mouse, Porcine, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51. AA range:21-70 |
| Concentration | 1 mg/ml |
| Dilution range | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 38 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-EIF2S1 antibody, anti-EIF2A antibody, anti-Eu Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
