EIF1AX Rabbit pAb Discontinued

SKU: orb373306

This product can be custom made

Description

Rabbit polyclonal antibody to EIF1AX

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	WB: 1:200-2000, IHC-P: 1:100-200
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 10-144 of human EIF1AX (NP_001403.1).
Molecular Weight	16kDa
Purity	Affinity purification
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EIF1A; EIF4C; eIF-1A; eIF-4C; EIF1AP1; EIF1AX

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunofluorescence analysis of C6 cells using EIF1AX Rabbit pAb (orb373306) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using EIF1AX Rabbit pAb (orb373306) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human stomach using EIF1AX Rabbit pAb (orb373306) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using EIF1AX Rabbit pAb (orb373306) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of various lysates using EIF1AX Rabbit pAb (orb373306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.