EGR4/EGR-4 Antibody

SKU: orb1536565

Description

EGR4/EGR-4 Antibody

Images & Validation

• Tested Applications: IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (5 µg/ml), WB (0.06 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: LS-B1525 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1525 was determined to be 5 ug/ml.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide from C-Terminus of human EGR4 (Q05215, NP_001956). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken (100%); Opossum (92%); Medaka, Drosophila, Water flea (90%); Xenopus (84%); Tamarin, Panda, Bat, Platypus (81%).
• Target: EGR4 / EGR-4
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose. PBS, 0.09% sodium azide, 2% sucrose
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

EGR4, AT133, Early growth response 4, NGFIC, NGFI-C, EGR-4, PAT133

Quick Database Links

Gene Symbol

EGR4 / EGR-4