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Catalog Number | orb1536565 |
---|---|
Category | Antibodies |
Description | EGR4/EGR-4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Synthetic peptide from C-Terminus of human EGR4 (Q05215, NP_001956). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken (100%); Opossum (92%); Medaka, Drosophila, Water flea (90%); Xenopus (84%); Tamarin, Panda, Bat, Platypus (81%). |
Concentration | 0.5 mg/ml |
Dilution range | IHC, IHC-P (5 µg/ml), WB (0.06 µg/ml) |
Conjugation | Unconjugated |
Target | EGR4 / EGR-4 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS, 0.09% sodium azide, 2% sucrose. PBS, 0.09% sodium azide, 2% sucrose |
Alternative names | EGR4, AT133, Early growth response 4, NGFIC, NGFI- Read more... |
Note | For research use only |
Application notes | Further information: Immunohistochemistry: LS-B1525 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1525 was determined to be 5 ug/ml. |
Expiration Date | 12 months from date of receipt. |