EGR1 Antibody (N-term)

SKU: orb34304

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Description

Purified rabbit polyclonal antibody.

Research Area

Cancer Biology, Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, WB
Dilution range	WB: 1:1000, FACS: 1:10-50, IF/ICC: 1:10-50
Reactivity	Human, Mouse
Predicted Reactivity	Other

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB19179
Immunogen	Synthetic Peptide
Target	EGR1
Molecular Weight	57507
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Anti-Early growth response protein 1 antibody, anti-EGR-1 antibody, anti-Protein Krox-24 antibody, anti-Transcription factor Zif268 antibody, anti-Nerve growth factor-induced protein A antibody, anti-NGFI-A antibody, anti-Transcription factor ETR103 antibody, anti-Zinc finger protein 225 antibody, anti-AT225 antibody, anti-ZNF225 antibody, anti-EGR1 antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of EGR1 Antibody (N-term) in mouse NIH-3T3 tissue lysates (35 ug/lane). EGR1 (arrow) was detected using the purified Pab.

EGR1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the EGR1 antibody detected the EGR1 protein (arrow).

Confocal immunofluorescent analysis of EGR1 Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

EGR1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

EGR1

UniProt

P18146

RefSeq (Protein):NP_001955.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001955.1

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence