EGLN1 Antibody, KO Validated

• Catalog Number: orb1274505
• Category: Antibodies
• Description: EGLN1 Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human EGLN1 (NP_071334.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 46kDa
• Target: EGLN1
• UniProt ID: Q9GZT9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: EGLN1, HPH2, PHD2, SM20, ECYT3, HPH-2, HIFPH2, ZMY
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EGLN1 antibody (orb1274505) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using EGLN1 antibody (orb1274505) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and EGLN1 knockout (KO) 293T cells, using EGLN1 antibody (orb1274505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.