EGFR (Phospho-Tyr1173) Rabbit mAb [KD Validated]

SKU: orb1727773

Description

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068.

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution range: WB:1:500~1:2000 IHC:1:50~1:100 ICC/IF:1:50~1:100 IP:1:20
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Phospho-EGFR (Y1173)
• Molecular Weight: 175kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Disclaimer: For research use only

Alternative Names

EC 2.7.10.1; ERBB1; Epidermal growth factor receptor precursor; kinase EGFR;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded rat stomach, using Phospho-EGFR (Y1197) Antibody.

Western blot analysis of Phospho-EGFR (Y1197) expression in A431 cell lysate treated with EGF.

Quick Database Links

UniProt

P00533