EGFR (phospho Tyr1016) rabbit pAb

• Catalog Number: orb767935
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGFR (phospho-Tyr1016)
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1016. AA range:986-1035
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 134
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-EGFR antibody, anti-ERBB antibody, anti-ERBB1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of HELA cells using Phospho-EGFR (Y1016) Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HeLa cells, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.