EGFR Antibody (Y1092)

SKU: orb1788319

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, IHC-P: 1:25
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1070-1099 amino acids from human EGFR. Antigen Region: 1070-1099 aa.
• Target: EGFR (HGNC:3236)
• Molecular Weight: 134277 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (Y1092). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from Hela, PC-3 cell line (from left to right), using EGFR-Y1092 Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

All lanes: Anti-EGFR Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: PC-3 whole cell lysates. Lane 3: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

EGFR (HGNC:3236)

UniProt

P00533

RefSeq (Protein):NP_958441.1, NP_958439.1, NP_958440.1, NP_005219.2