EGF Receptor / EGFRvIII Antibody

SKU: orb606694

Description

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Titering of the recombinant EGFRvIII antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: GFR/2600R
• Immunogen: Amino acids LEEKKGNYVVTDHC were used as the immunogen for the recombinant EGFRvIII antibody. This is the predicted sequence of the exon 2-7 delete fusion region.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human lung squamous cell carcinoma with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.

IHC testing of FFPE human placenta with recombinant EGFRvIII antibody (clone GFR/2600R). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool prior to testing.

SDS-PAGE analysis of purified, BSA-free recombinant EGFRvIII antibody (clone GFR/2600R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P00533