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Catalog Number | orb1231216 |
---|---|
Category | Proteins |
Description | EGF R Recombinant Protein |
Target | EGFR |
Tag | His Tag |
Form/Appearance | Lyophilized |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | > 95% as determined by SDS-PAGE. |
Protein Sequence | Leu 25 - Ser 645 |
UniProt ID | P00533 |
MW | 70.5kDa |
Tested applications | ELISA, WB |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Source | HEK293 cells |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Alternative names | EGFR,ERBB,ERBB1,HER1,PIG61,mENA |
Note | For research use only |
NCBI | NP_005219.2 |
Human EGF R, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Cetuximab at 2 ug/ml (100 uL/well) can bind Human EGF R, His Tag, low endotoxin with a linear range of 0.8-6 ng/mL.
ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
94.7 kDa | |
HEK293 cells |
> 97% as determined by SDS-PAGE and HPLC. | |
9.7 kDa | |
E.Coli |
Greater than 85% as determined by SDS-PAGE. | |
11.7 kDa | |
Yeast |