EGF R Recombinant Protein

• Catalog Number: orb1231148
• Category: Proteins
• Description: EGF R Recombinant Protein
• Target: EGFR
• Tag: Fc Tag
• Form/Appearance: Lyophilized
• Buffer/Preservatives: PBS, pH 7.4
• Purity: > 95% as determined by SDS-PAGE.
• Protein Sequence: Leu 25 - Ser 645
• UniProt ID: P00533
• MW: 94.7 kDa
• Tested applications: ELISA, WB
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Source: HEK293 cells
• Storage: Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
• Alternative names: EGFR,ERBB,ERBB1,HER1,PIG61,mENA
• Note: For research use only
• NCBI: NP_005219

Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human EGF, Tag Free at 10 ug/ml (100 uL/well) can bind Human EGF R, Fc Tag with a linear range of 0.078-2.5 ug/ml.

Immobilized Human EGF R, Fc Tag at 1 ug/ml (100 uL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL.