Eg5 rabbit pAb

• Catalog Number: orb765106
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Eg5
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human KIF11/Eg5. AA range:892-941
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 119
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-KIF11 antibody, anti-EG5 antibody, anti-KNSL1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Eg5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western Blot analysis of COLO205 cells using Eg5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HeLa cells, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.