EFTUD2 Antibody

SKU: orb1256642

Description

EFTUD2 Antibody

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
• Target: EFTUD2
• Molecular Weight: Observed: 120kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

116 kDa U5 small nuclear ribonucleoprotein component, Elongation factor Tu GTP-binding domain-containing protein 2, SNU114 homolog, hSNU114, U5 snRNP-specific protein, 116 kDa, U5-116 kDa, EFTUD2, KIAA0031, SNRP116

Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (orb1256642) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.

Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using EFTUD2 antibody (orb1256642).

Immunoprecipitation analysis of 100 ug extracts of 293T cells using 3 ug EFTUD2 antibody (orb1256642). Western blot was performed from the immunoprecipitate using EFTUD2 antibody (orb1256642) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

EFTUD2

UniProt

Q15029