EFTUD2 Antibody

• Catalog Number: orb1256642
• Category: Antibodies
• Description: EFTUD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 120kDa
• Target: EFTUD2
• UniProt ID: Q15029
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: 116 kDa U5 small nuclear ribonucleoprotein compone
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (orb1256642) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.

Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody (orb1256642) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using EFTUD2 antibody (orb1256642).

Immunoprecipitation analysis of 100 ug extracts of 293T cells using 3 ug EFTUD2 antibody (orb1256642). Western blot was performed from the immunoprecipitate using EFTUD2 antibody (orb1256642) at a dilution of 1:1000.