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EFNB2/Ephrin B2 Antibody

SKU: orb1537179

Description

EFNB2/Ephrin B2 Antibody

Images & Validation

Tested ApplicationsELISA, IHC-P, WB
Dilution RangeIHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
ReactivityBovine, Canine, Equine, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rat
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 35kD band observed in Human, Mouse and Rat Lung lysates (calculated MW of 36.9kD according to NP_004084.1). Recommended concentration: 0.1-0.3 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence CPKVDSKTVGQYE, from the internal region of the protein sequence according to NP_004084.1.
TargetEFNB2 / Ephrin B2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

EFNB2, Ephrin-B2, EPLG5, Ephrin B2, HTK ligand, Htk-L, LERK-5, LERK5, Ligand of eph-related kinase 5, HTKL

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EFNB2/Ephrin B2 Antibody

EFNB2 antibody (0.1 ug/ml) staining of Rat Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

EFNB2/Ephrin B2 Antibody (orb1537179)

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50 μg
$ 640.00
DispatchUsually dispatched within 2-3 weeks
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