EFEMP1 Antibody (N-term)

• Catalog Number: orb1928113
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22140
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54641 Da
• Target: This EFEMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the N-terminal region of human EFEMP1.
• UniProt ID: Q12805
• NCBI: NP_001034438.1, NP_001034437.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: EGF-containing fibulin-like extracellular matrix p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EFEMP1 Antibody (N-term) in mouse lung tissue lysates (35 ug/lane). EFEMP1 (arrow) was detected using the purified Pab.

EFEMP1 Antibody (N-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EFEMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-EFEMP1 Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.