EEF2 Rabbit mAb [KD Validated]

• Catalog Number: orb1727398
• Category: Antibodies
• Description: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human EEF2
• Antibody Type: Recombinant Antibody
• Dilution range: WB:1:1000~1:5000IHC:1:50~1:200ICC/IF:1:50~1:200IP:1:20FC:1:50
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• MW: 95kDa
• UniProt ID: P13639
• Source: Recombinant Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EEF 2; Eef2; EF-2; EF2; Elongation factor 2; Polyp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Western blot analysis of EEF2 expression in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.