EEF1E1 Mouse Monoclonal Antibody

SKU: orb783007

Description

EEF1E1 Mouse Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: WB=1:500-4000, ICC/IF=1:25, Flow-Cyt=1:25
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone No.: BI5G6
• Immunogen: Recombinant human EEF1E1.
• Target: EEF1E1
• Molecular Weight: 18 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AIMP3; P18; 1110003A02Rik; MCA3_HUMAN; EEF1E1; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; Elongation factor p18; Multisynthase complex auxiliary component p18; MCA3_MOUSE;

Blank control: Hela. Primary Antibody (green line): Mouse Anti-EEF1E1 antibody (orb783007), dilution: 1:25, Isotype Control Antibody (blue line): Mouse IgG, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1:400, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EEF1E1) monoclonal Antibody, Unconjugated (orb783007) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, Dylight® 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin. The nuclear counter stain is DAPI (blue).

Sample: Lane 1: HepG2 cell lysates, Lane 2: Human testis tissue lysates, Lane 3: A549 cell lysates, Lane 4: A431 cell lysates, Lane 5: Jurkat cell lysates, Lane 6: Mouse brain tissue lysates, Primary: Anti-EEF1E1 (orb783007) at 1/2000~4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 18 kD.

Quick Database Links

Gene Symbol

EEF1E1

UniProt

O43324