EEF1D Antibody

• Catalog Number: orb1258142
• Category: Antibodies
• Description: EEF1D Antibody
• Target: EEF1D
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).
• UniProt ID: P29692
• MW: Observed: 38kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EF-1D, EF1D, FP1047, elongation factor 1-delta, EF
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of U2OS cells using EEF1D antibody (orb1258142). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EEF1D antibody (orb1258142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using EEF1D antibody (orb1258142) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EEF1D antibody (orb1258142) at dilution of 1:200 (40x lens).