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Catalog Number | orb1258142 |
---|---|
Category | Antibodies |
Description | EEF1D Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 38kDa |
Target | EEF1D |
UniProt ID | P29692 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | EF-1D, EF1D, FP1047, elongation factor 1-delta, EF Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of U2OS cells using EEF1D antibody (orb1258142). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using EEF1D antibody (orb1258142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using EEF1D antibody (orb1258142) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EEF1D antibody (orb1258142) at dilution of 1:200 (40x lens).
IF, IHC, WB | |
Human, Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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