EEA1 Antibody

• Catalog Number: orb1248147
• Category: Antibodies
• Description: EEA1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-QETKIQHEELNNRIQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 200kDa band observed in lysates of cell line HEK293 (calculated MW of 162kDa according to NP_003557.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubatiion 1 hour at room temperature. Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 200kDa
• Target: EEA1
• UniProt ID: Q15075
• NCBI: NP_003557.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: EEA1, early endosome antigen 1, MST105, MSTP105, Z
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 200kDa band observed in lysates of cell line HEK293 (calculated MW of 162kDa according to NP_003557.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubatiion 1 hour at room temperature. Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248147 (0.3 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248147 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle/cytoplasmic staining.