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Item 1 of 4
Item 1 of 4
EDG6 Antibody (N-term)
Catalog Number: orb1930229
| Catalog Number | orb1930229 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB1829 |
| Tested applications | WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:500, WB: 1:1000, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 41623 Da |
| Target | This EDG6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human EDG6. |
| UniProt ID | O95977 |
| NCBI | NP_003766.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Sphingosine 1-phosphate receptor 4, S1P receptor 4 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
EDG6 Antibody (R23) western blot analysis in NCI-H292 cell line lysates (35 ug/lane). This demonstrates the EDG6 antibody detected the EDG6 protein (arrow).
EDG6 Antibody (R23) western blot analysis in mouse spleen tissue lysates (35 ug/lane). This demonstrates the EDG6 antibody detected the EDG6 protein (arrow).
All lanes: Anti-EDG6 Antibody (N-term) at 1:500 dilution. Lane 1: NCL-H292 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-EDG6 Antibody at 1:1000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size: 47kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
