EDC3 phospho S161 Antibody

SKU: orb345760

Description

EDC3 pS161 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1: 10,000, WB: 0.5ug/ml
• Reactivity: Mouse
• Application Notes:
  Anti-EDC3pS161 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~55kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: EDC3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide surrounding the pS161 site of mouse EDC3.
• Purity: Anti-EDC3 pS161 is specific for phospho S161 mouse EDC3. It was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with rabbit, pig, monkey, bovine, rat, mouse, and bat based on 100% sequence homology. Cross-reactivity with EDC3 pS161 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-EDC3 pS161 Antibody, Enhancer of mRNA-decapping protein 3, YjeF domain-containing protein 1, Edc-3, Edc 3, Yjdc

Western Blot and Immunofluorescence of Anti-EDC3 phosho S161 status in P‐bodies. A, B. Western blot of whole cell lysates (WCL) and P‐body (PB) purified from PC3‐LN4 cells stably expressing GFP or GFP‐DCP1a used as a P‐body tag. The P‐bodies (PB) were isolated with GFP‐Trap beads and analyzed by Western blotting with the indicated Abs. C. Immunofluorescence (IF) of PC3LN4 cells under normal culture condition with anti‐phospho‐EDC3, anti‐EDC3 Abs, and DAPI. Images captured under 40× magnification; scale bar: 10 µm.

Western blot of Anti-EDC3 phospho S161 Antibody. EDC3 S161 influences cellular growth and migration. A. Western blot of lysates prepared from CRISPR/Cas9 PC3‐LN4 EDC3 KO (#1, 2) cells, EDC3 S161A (#1, 2), EDC3 S161D (#1, 2) mutants, and parental cells with the indicated Abs. B. PC3‐LN4 EDC3 KO cells containing doxycycline (DOX)‐inducible EDC3 WT, S161A, or vector control (Mock) were treated for 48 h with 0, 75, and 150 ng/mL DOX. All extracts were subjected to Western blotting with the indicated Abs.

Western blot of Anti-EDC3 phospho S161 Antibody. P‐EDC3 levels regulate tumor growth and are elevated in multiple breast cancer cell lines. B. Phosphorylated EDC3 levels were examined in normal immortalized breast cell lines, MCF10A and MCF12A, human breast cancer cell lines HCC38, HCC1954, MDA‐MB‐231, MDA‐MD‐468, BT‐549, MRKNU1, and mouse 4T1 by Western blotting with the indicated Abs.

Quick Database Links

UniProt

Q8K2D3