EDA Antibody (N-term)

• Catalog Number: orb1930125
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13883
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 41294 Da
• Target: This EDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human EDA.
• UniProt ID: Q92838
• NCBI: NP_001005612.2, NP_001005613.1, NP_001005609.1, NP_001390.1, NP_001005610.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ectodysplasin-A, Ectodermal dysplasia protein, EDA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of hela cells using EDA Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of lysate from RD cell line, using EDA Antibody (N-term).Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.