ECSM2 Antibody

• Catalog Number: orb1248311
• Category: Antibodies
• Description: ECSM2 Antibody
• Target: ECSM2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-NNGKQSLSAEKVL
• UniProt ID: Q19T08
• MW: Approx 50kDa
• Tested applications: ELISA, IF, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ref PMID: 18556573). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Vessel. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470.Immunofluorescence: This product has been successfully used in IF on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: endothelial cell-specific marker 2, endothelial ce
• Research Area: Cancer Biology, Immunology & Inflammation, Neurosc
• Note: For research use only
• NCBI: NP_001071161.1

orb1248311 (1 ug/ml) staining of Human lung (A) and Human Umbilical Cord (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour.

orb1248311 (5 ug/ml) staining of paraffin embedded Human Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.