ECSM2 Antibody

SKU: orb1248311

Description

ECSM2 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies. Recommended concentration: 1-3ug/ml. Immunohistochemistry: Paraffin embedded Human Vessel. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Human. Immunofluorescence: This product has been successfully used in IF on Human.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NNGKQSLSAEKVL
• Target: ECSM2
• Molecular Weight: Approx 50kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

endothelial cell-specific marker 2, endothelial cell-specific molecule 2, hypothetical protein LOC641700

orb1248311 (1 ug/ml) staining of Human lung (A) and Human Umbilical Cord (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour.

orb1248311 (5 ug/ml) staining of paraffin embedded Human Vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ECSM2

UniProt

Q19T08

RefSeq (Protein):NP_001071161.1