ECSIT Antibody (C-term)

• Catalog Number: orb1935421
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34997
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 49148 Da
• Target: This ECSIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human ECSIT.
• UniProt ID: Q9BQ95
• NCBI: NP_001135937.1, NP_001135936.1, NP_057665.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Evolutionarily conserved signaling intermediate in
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ECSIT Antibody (C-term) western blot analysis in Jurkat, ZR-75-1 cell line lysates (35 ug/lane). This demonstrates the ECSIT antibody detected the ECSIT protein (arrow).

Fluorescent confocal image of Hela cell stained with ECSIT Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ECSIT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ECSIT immunoreactivity is localized to Cytoplasm and Nucleus significantly.